Establishment of a leukemic cell model for studying human pre-B to B cell differentiation.

摘要: Reproducible models for examining early stages of human B cell differentiation are poorly developed. We now describe the establishment and characterization a novel leukemic line that recapitulates pre-B to stage differentiation. This line, designated BLIN-1, was initially established in tissue culture medium containing low m.w. growth factor, consistently shows dependency on this cytokine optimal at density. BLIN-1 cells have 9p chromosomal abnormality, identical abnormality present blasts from patient's original bone marrow aspirate. The immunologic phenotype is consistent with arrested development. Analysis Ig gene rearrangement expression series subclones show spontaneously rearrange kappa light chain genes, leading surface kappa-negative into kappa-positive cells. is, our knowledge, first defined model critical developmental ontogeny. As such, it offers unique resource variables influencing onset L expression.