The Goldilocks Approach: A Review of Employing Design of Experiments in Prokaryotic Recombinant Protein Production

摘要: The production of high yields soluble recombinant protein is one the main objectives biotechnology. Several factors, such as expression system, vector, host, media composition and induction conditions can influence yield. Identifying most important factors for optimum may involve significant investment time considerable cost. To address this problem, statistical models Design Experiments (DoE) have been used to optimise production. This review examines application DoE in proteins prokaryotic systems with specific emphasis on culture conditions. commonly screening optimisation designs. It provides examples applied