Endothelial Intercellular Adhesion Molecule-1 Expression Is Suppressed in Human Malignancies: The Role of Angiogenic Factors
作者： Stefano Martinotti , Cora A. Damen , Gerard Groenewegen , Arjan W. Griffioen , Geert H. Blijham
DOI:
关键词:
摘要: Intercellular adhesion molecule 1 (ICAM-1) is involved in the recirculation of blood leukocytes and, presumably, infiltration cytolytic effector into tumors. The present report describes a down-regulated expression vascular ICAM-1 on tumor-infiltrating endothelial cells (EC) renal cell carcinoma. This finding was obtained by flow cytometric analysis tumor EC compared to from healthy tissue. Since growth solid tumors dependent formation new vessels (angiogenesis), we hypothesized that angiogenic factors are responsible for down-regulation ICAM-1. hypothesis investigated vitro using human umbilical vein- and dermis-derived EC. Using cytometry, found biphasic regulation during stimulation cultured with agent basic fibroblast factor (bFGF). Although 16-24 h after activation marked up-regulation observed, significantly decreased 48h. longevity this at least 7 days. Northern blot revealed steady-state mRNA level gene. ICAM-2 showed similar results intial up- later down-regulation. Functional relevance changes demonstrated corresponding EC-leukocyte bFGF. described effects specific bFGF since other (such as factor, transforming beta, interleukin 8) did not affect expression. Subsequent experiments decrease sensitivity necrosis factor-alpha regard reveal tumor-derived escape mechanism molecules vivo responsiveness proinflammatory cytokines.
aacrjournals.org
researchgate.net 参考文章(23)
MG Lampugnani, M Resnati, E Dejana, A Aiello, E Fontanella, D Soligo, MA Pierotti, MF Greaves, D Delia, CD34 expression is regulated reciprocally with adhesion molecules in vascular endothelial cells in vitro. Blood. ,vol. 81, pp. 1001- 1008 ,(1993) , 10.1182/BLOOD.V81.4.1001.1001
Stefania Uccini, Carlo D. Baroni, Aldo Tagliabue, Diana Boraschi, Rod Pigott, Antonella Stoppacciaro, Luigi P. Ruco, Francesca Monardo, Donatella Pomponi, Angela Santoni, Alberto Mantovani, Elisabetta Dejana, Cytokine Production (IL-1α, IL-1β, and TNFα) and Endothelial Cell Activation (ELAM-1 and HLA-DR) in Reactive Lymphadenitis, Hodgkin's Disease, and in Non-Hodgkin's Lymphomas: An Immunocytochemical Study American Journal of Pathology. ,vol. 137, pp. 1163- 1171 ,(1990)
L Fina, HV Molgaard, D Robertson, NJ Bradley, P Monaghan, D Delia, DR Sutherland, MA Baker, MF Greaves, Expression of the CD34 gene in vascular endothelial cells. Blood. ,vol. 75, pp. 2417- 2426 ,(1990) , 10.1182/BLOOD.V75.12.2417.2417
R. O. Schlingemann, F. J. R. Rietveld, D. J. Ruiter, A. J. S. Davies, M. F. Greaves, A. I. Skene, N. J. Bradley, J. Denekamp, R. M. W. De Waal, Leukocyte antigen CD34 is expressed by a subset of cultured endothelial cells and on endothelial abluminal microprocesses in the tumor stroma. Laboratory Investigation. ,vol. 62, pp. 690- 696 ,(1990)
R. O. Schlingemann, F. J. R. Rietveld, F. Kwaspen, P. C. M. Van De Kerkhof, D. J. Ruiter, R. M. W. De Waal, Differential expression of markers for endothelial cells, pericytes, and basal lamina in the microvasculature of tumors and granulation tissue. American Journal of Pathology. ,vol. 138, pp. 1335- 1347 ,(1991)
Intercellular adhesion molecule-1 is a cell surface receptor for hyaluronan. Journal of Biological Chemistry. ,vol. 269, pp. 30081- 30084 ,(1994) , 10.1016/S0021-9258(18)43775-1
E. Caldenhoven, P. T. Van Der Saag, A. Van De Stolpe, F. Horn, W. Kruijer, Juping Yuan, P. Coffer, Stimulation of the human intercellular adhesion molecule-1 promoter by interleukin-6 and interferon-gamma involves binding of distinct factors to a palindromic response element. Journal of Biological Chemistry. ,vol. 269, pp. 21146- 21154 ,(1994) , 10.1016/S0021-9258(17)31942-7
A. van de Stolpe, E. Caldenhoven, B.G. Stade, L. Koenderman, J.A. Raaijmakers, J.P. Johnson, P.T. van der Saag, 12-O-tetradecanoylphorbol-13-acetate- and tumor necrosis factor alpha-mediated induction of intercellular adhesion molecule-1 is inhibited by dexamethasone. Functional analysis of the human intercellular adhesion molecular-1 promoter. Journal of Biological Chemistry. ,vol. 269, pp. 6185- 6192 ,(1994) , 10.1016/S0021-9258(17)37586-5
Hirokazu Nagawa, Wataru Kimura, Joji Kitayama, Nelson Tsuno, Yoichi Shibata, Hiroshi Yasuhara, Tetsuichiro Muto, Suppressive Effect of Basic Fibroblast Growth Factor on Transendothelial Emigration of CD4(+) T-Lymphocyte Cancer Research. ,vol. 54, pp. 4729- 4733 ,(1994)
T. J. Lawley, R. A. Swerlick, Lian-Jie Li, N. T. Sepp, S. W. Caughman, K. H. Lee, Regulation of vascular cell adhesion molecule 1 on human dermal microvascular endothelial cells. Journal of Immunology. ,vol. 149, pp. 698- 705 ,(1992)