Characterization of the CCR2 chemokine receptor: functional CCR2 receptor expression in B cells.

摘要: We have derived anti-human CCR2-specific mAbs by immunization with synthetic peptides corresponding to CCR2 sequences presumably involved in the interaction its ligand(s). The characterization of these includes ability recognize receptor specifically, as well function based on their promote Ca2+ influx or block MCP-1-induced and chemotaxis. One mAb (MCP-1 R02) that is directed NH2 terminal domain has MCP-1 agonist activity, two third extracellular (MCP-1R04 R05) antagonist activity. analyzed presence several PBL tonsil-derived leukocyte populations found expression this monocytes, activated T cells, and, surprisingly, B cells. cells was further corroborated Southern blot using probes. Moreover, both trigger specific cell migration via a PTX-sensitive mechanism, indicating functional