Application of surface plasmon resonance for the detection of carbohydrates, glycoconjugates, and measurement of the carbohydrate-specific interactions: A comparison with conventional analytical techniques. A critical review

摘要: Carbohydrates (glycans) and their conjugates with proteins lipids contribute significantly to many biological processes. That makes these compounds important targets be detected, monitored identified. The identification of the carbohydrate content in (glycoforms) is often a challenging task. Most conventional instrumental analytical techniques are time-consuming require tedious sample pretreatment utilising various labeling agents. Surface plasmon resonance (SPR) has been intensively developed during last two decades received increasing attention for different applications, from real-time monitoring affinity bindings biosensors. SPR does not any labels capable direct measurement biospecific interaction occurring on sensing surface. This review provides critical comparison modern terms capabilities detect carbohydrates, study carbohydrate-specific bindings. A few selected examples approaches 2004-2011 biosensing glycoforms glycan-protein studies comprehensively discussed.