作者: Robert E. Hawkins , Greg Winter , Terry J. Hamblin , Freda K. Stevenson , Stephen J. Russell
DOI: 10.1097/00002371-199311000-00004
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摘要: Treatment of cancer with vaccines is an attractive prospect, but few tumours express suitable target antigens. With B-cell lymphomas, the idiotypic immunoglobulin (Ig) malignant should provide a this requires vaccine to be created for each patient. We propose strategy making such vaccines: first clone V genes Ig, and second inject patient cloned DNA (genetic immunisation) in order elicit immune response against encoded Ig. have previously shown that Ig can identified from human lymph node biopsies by polymerase chain reaction amplification, cloning, sequencing. In report, we show anti-idiotypic antibodies elicited direct injection expression vector encodes murine (the B1.8, hybridoma or BCL1, lymphoma line). This finding suggests simple approach preparation patients lymphoma, which also circumvents need adjuvants.