Expanded repertoire of kinetoplast associated proteins and unique mitochondrial DNA arrangement of symbiont-bearing trypanosomatids

摘要: In trypanosomatids, the kinetoplast is portion of single mitochondrion that connected to basal body and contains kDNA, a network composed by circular interlocked DNA. The kDNA packing conducted Kinetoplast Associated Proteins (KAPs), which are similar eukaryotic histone H1. symbiont-harboring trypanosomatids (SHTs) such as Angomonas deanei Strigomonas culicis, s-proteobacterium co-evolves with host in mutualistic relationship. prokaryote confers nutritional benefits affects its cell structure. Atomic force microscopy showed topology isolated networks quite two SHT species. Ultrastructural analysis using high-resolution techniques revealed DNA fibrils more compact region faces presence symbiotic bacterium does not interfere topology. However, RT-PCR data differences expression KAPs wild-type protozoa compared aposymbiotic cells. Immunolocalization different present distinct distributions coincident symbiont-bearing symbiont-free Although KAP4 KAP7 shared all trypanosomatid species, expanded repertoire SHTs can be used phylogenetic markers distinguish genera.