Eel osmotic stress transcriptional factor 1 (Ostf1) is highly expressed in gill mitochondria-rich cells, where ERK phosphorylated

摘要: Background: Osmotic stress transcriptional factor 1 (Ostf1) was firstly identified in tilapia 2005. Then numerous studies have investigated its regulation and expression profile fish gill tissues related to osmoregulation. Generally, hyperosmotic induced ostf1 mRNA level, however there is no report studying the cellular localization of Ostf1 any osmoregulatory tissue. In this study immunohistochemical (IHC) approach used cells Japanese eels. Findings: protein found be localized branchial mitochondria-rich/chloride cell (MRC/CC) as revealed by Naa5 CFTR co-localization. The detectable at day 3 after fresh water seawater transfer mainly MRCs. Moreover, elevated levels extracellular signal regulated kinase (ERK) phosphorylation observed co-localized with Conclusions: Our data observation supports role osmosensing and/or osmoregulation gills, particularly functional relationship co-expression pERK MRCs suggests a cross-talk mechanism between mitogen-activated kinases (MAPKs) response challenge. To summarize, has addressed provides evidence illustrate involvement ERK on function