Transcriptomic Analysis of Trout Gill Ionocytes in Fresh Water and Sea Water Using Laser Capture Microdissection Combined with Microarray Analysis

摘要: Fish gills represent a complex organ composed of several cell types that perform multiple physiological functions. Among these cells, ionocytes are implicated in the maintenance ion homeostasis. However, because ionocyte represents only small percent whole gill tissue, its specific transcriptome can be overlooked among numerous included gill. The objective this study is to better understand functions by comparing RNA expression type freshwater and seawater acclimated rainbow trout. To realize objective, were captured from cryosections using laser capture microdissection after immunohistochemistry. Then, analyses performed on an Agilent trout oligonucleotide microarray. Gene analysis identified 108 unique annotated genes differentially expressed between ionocytes, with fold change higher than 3. Most up-regulated cells. Interestingly, transport, extracellular matrix structural cellular proteins appeared ionocytes. them, transporters, such as CIC2, SLC26A6, NBC, validated qPCR and/or situ hybridization. latter technique allowed us localize transcripts transporters more particularly Genes involved metabolism also transcriptional regulation, signaling cycle enhanced In conclusion, combined microarray for determination signature scarce cells fish gills, aided characterization