Responses of gill mitochondria-rich cells in Mozambique tilapia exposed to acidic environments (pH 4.0) in combination with different salinities

摘要: Abstract On exposure to hyposmotic acidic water, teleost fish suffer from decreases in blood osmolality and pH, consequently activate osmoregulatory acid–base regulatory mechanisms restore disturbed ion balances. In Mozambique tilapia Oreochromis mossambicus exposed (pH 4.0) or neutral 7.4–7.7) freshwater combination with 0 mM 50 mM NaCl, we examined functional morphological changes gill mitochondria-rich (MR) cells. We assessed gene expression of Na + /H exchanger-3 (NHE3), /Cl − cotransporter (NCC), vacuolar-type H -ATPase (V-ATPase) /HCO 3 cotransporter-1 (NBC1) the gills. The mRNA NHE3 NCC gills were higher than that supplemented while there was no significant difference levels V-ATPase NBC1. addition, immunocytochemical observations showed apical-NHE3 MR cells enlarged, frequently formed multicellular complexes developed deep apical openings NaCl. These findings suggest respond external salinity pH treatments, by parallel manipulation mechanisms.