Comparative transcriptome analysis reveals osmotic-regulated genes in the gill of Chinese mitten crab (Eriocheir sinensis)

摘要: Salinity is one of the most important abiotic factors directly affecting reproduction, molting, growth, immune, physiological and metabolic activities Chinese mitten crab (Eriocheir sinensis). This species has strong osmoregulatory capacity can maintain stringent internal homeostasis. However, mechanisms conferring tolerance to salinity fluctuations are not well understood. To reveal genes pathways involved in osmoregulation, adult male crabs (body weight = 110 ± 5 g) were acclimated for 144 h freshwater (FW, 0 ppt) or seawater (SW, 25 ppt). Changes transcriptome gills then analysed by RNA-Seq, 174,903 unigenes obtained. Comparison between FW- SW-acclimated groups identified 932 that significantly differentially expressed gill, comprising 433 499 up- downregulated transcripts. Gene Ontology functional enrichment analysis revealed biological processes related salt stress enriched, including energy metabolism, ion transport, signal transduction antioxidant activity. Kyoto Encyclopaedia Genes Genomes mapped 241 specific pathways, oxidative phosphorylation tricarboxylic acid (TCA)/citrate cycle enriched. altered expression many enzymes citrate synthase (CS), Na+/K+-ATPase (NKA), Na+-K+-2Cl cotransporter-1 (NKCC1), dopamine receptor D1 (DRD1), synaptic binding protein 1 (STXBP1), Cu2+/Zn2+ superoxide dismutase (SOD1) glutathione S-transferase (GST). Additionally, obtained transcriptomic sequencing data provided a useful resource identification novel genes, further crab.