BMAL1 controls the diurnal rhythm and set point for electrical seizure threshold in mice.

摘要: The epilepsies are a heterogeneous group of neurological diseases defined by the occurrence unprovoked seizures which, in many cases, correlated with diurnal rhythms. In order to gain insight into biological mechanisms controlling this phenomenon, we characterized time-of-day effects on electrical seizure threshold mice. Male C57BL/6J wild-type mice were maintained 14h/10h light/dark cycle, from birth until 6 weeks age for testing. Seizure thresholds measured using step-wise paradigm involving single daily stimulus. Results showed that current required elicit both generalized and maximal was significantly higher tested during dark phase cycle compared light phase. This rhythm absent BMAL1 knockout (KO) KO also exhibited reduced at all times tested, document significant influence suggest effect is under control genes known regulate circadian behaviors. Furthermore, low itself directly involved neuronal excitability.