Prevalence and molecular typing of Metallo-β-lactamase-producing Pseudomonas aeruginosa with adhesion factors: A descriptive analysis of burn wounds isolates from Iran

摘要: Abstract Pseudomonas aeruginosa produces a wide range of adhesion factors involved in pathogenicity and antibiotic resistance. The aim this study was to determine the prevalence Metallo-β-lactamase (MBL) enzymes virulence among clinical isolates. In Descriptive-analytical study, 73 samples collected from post-burn infection patients. MBL-producing strains were detected by phenotypic methods. All isolates investigated for presence β-lactamase-producing factor genes. identification MBL enzyme present revealed 20 (27.39%) modified Hodge test (MHT) Carba-NP methods, 21 (28.76%) Carbapenem Inactivation Method 19 (26.02%) (mCIM) method. Moreover, 21(28.76%) considered as strong biofilms, five (6.84%) medium biofilms. β-lactamase genes P. follows: blaSMP (9.58%), blaVIM (21.91%), blaIMP (16.43%), blaKPC (23.28%), blaSIM (12.32%), blaNDM (6.84%), blaGIM (9.58%). addition, pclN (49.31%), toxA (79.45%), exoS (72.6%), phzM (39.72%), pclH lasB (50.68%), apr (30.13%), lasA (56.16%), nanL (23.28%) algD (54.79%). However, significant statistical relationship reported between enzyme, extracellular genes, biofilm production. results showed that isolated plays an important role increasing Likewise, plasmids carrying are diverse stable multidrug resistance (MDR) extensively drug-resistant (XDR)