Coexistence of Virulence Factors and Efflux Pump Genes in Clinical Isolates of Pseudomonas aeruginosa: Analysis of Biofilm-Forming Strains from Iran

摘要: Background. Biofilm formation and efflux pumps (EPs) correlation play a critical role in the pathogenicity antibiotic resistance of Pseudomonas aeruginosa. In this study, biofilm EP’s collaborative clinical isolates P. aeruginosa infection were investigated. Methods. Eighty-six (86) collected from different specimens confirmed using biochemical tests. The was investigated by crystal violet assay. Also, EP genes identified PCR method. Results. Based on results, gentamicin-resistant (n = 50, 66.29%) ciprofloxacin-resistant (n = 61, 69.66%) strains most frequent colistin (n = 1, 1.12%) ceftazidime (n = 12, 7.86%) resistant least prevalent. Furthermore, 22 (31.42%) MDR, 11 (12.35%) XDR strains. 19 (22.47%) classified as strong biofilm, 29 (21.34%) moderate 3 (11.23%) weak producers. distribution follows: mexA (n = 44, 34.83%), mexB (n = 33, 32.58%), oprM (n = 59, 29.21%), oprD 30.33%), tetA (n = 22, 25.58%), tetR (n = 19, 22.09%), emrE (n = 21, 24.41%). However, there significant association between EPs Conclusions. we suggested that presence multidrug pump, MexEF-OprN, significantly reduced pathogenicity. contrast, MexAB-OprM MexCD-OprJ did not affect virulence.