RND type efflux pump system MexAB-OprM of Pseudomonas aeruginosa selects bacterial languages, 3-oxo-acyl-homoserine lactones, for cell-to-cell communication.

摘要: Bacteria release a wide variety of small molecules including cell- to- cell signaling compounds. Gram-negative bacteria use self-produced autoinducers such as acylated homoserine lactones (acyl- HSLs) signal compounds for quorum sensing (QS) within and between bacterial species. QS plays significant role in the pathogenesis infectious diseases beneficial symbiosis by responding to acyl- HSLs Pseudomonas aeruginosa. It is considered that selection languages necessary regulate gene expression thus it leads regulation virulence provides growth advantage several environments. In this study, we hypothesized RND-type efflux pump system MexAB- OprM P. aeruginosa might function HSLs, provide evidence support hypothesis. Loss due deletion mexB caused increases responses, shown gfp located downstream lasB promoter LasB elastase activity, which regulated LasR- 3- oxo- C12- HSL complex. Either complementation with plasmid containing wild- type or addition specific inhibitor, patulin, repressed these high responses HSLs. Furthermore, was HSLs- dependent response affected inhibition MexB transport activity mutant. The mutant showed strong C10- produced Vibrio anguillarum cross- talk experiment. This work demonstrated does not control binding LasR 3-oxo-Cn-HSLs but rather accessibility non-cognate acyl-HSLs only influences multidrug resistance, also selects regulates results demonstrate new mechanism via