Micropropagation of Vegetable Rennet (Withania coagulans[Stocks] Dunal)—A Critically Endangered Medicinal Plant

摘要: Withania coagulans (Stocks) Dunal (Solanaceae), popularly called vegetable rennet, is a critically endangered and highly valued medicinal plant. Overexploitation reproductive failure forced the plant species toward verge of complete extinction. We describe here development simple, rapid, cost effective in vitro micropropagation system for W. mass-scale production true-to-type plantlets using nodal shoot segments. Exactly 95.5 ± 0.34% explants responded within 8–10 days (d) produced multiple buds (4.1 0.10 shoots 2.95 0.15 cm length) on 0.8% agar-gelled Murashige Skoog's (MS) basal medium supplemented with 8.88 μM 6-benzylaminopurine (BAP), 0.57 indole-3-acetic acid (IAA), additives (100 mg L−1 L-ascorbic acid, 25 each citric adenine sulphate, L-arginine). The cultures were multiplied by repeated transfer MS 4.44 BAP, IAA, additives. Further large-scale th...