An improved thin cell layer culture system for efficient clonal propagation and in vitro withanolide production in a medicinal plant Withania coagulans Dunal

摘要: Abstract In vitro micropropagation based on transverse thin cell layer (tTCL), which utilizes a very small number of cells or tissues, has now emerged as powerful tool in plant tissue differentiation and regeneration. this study, intense, fast rate shoot multiplication been achieved utilizing explants for endangered pharmaceutical Withania coagulans Dunal (family Solanaceae) having high hypoglycemic potential. To develop an efficient protocol rapid propagation W. stem node, apical meristem (SAM), tTCL were used inoculated different strength MS medium. The explant showed frequency regeneration was also affected by the concentration growth regulators. full solid medium optimal (31.94 ± 1.39%) highest percent shoots (93.05 ± 2.77) observed fortified with 2.0 mg l−1 BAP 0.5 mg l−1 NAA. contrast to tTCL, SAM cultured same significantly lower only 1.16 ± 0.08 average per responsive explants. best rooting regenerated half liquid supplemented 2 mg l−1 IBA. Plants successfully transferred field after acclimatization survival 90.6%. Monomorphic nature ISSR RAPD markers study confirmed genetic fidelity raised clones. Quantification withanolides content through performance chromatography (HPLC) 1.4 fold increases withaferin A 1.6 withanolide acclimatized grown plants compared (wild type) plants. over-expression SQS gene further attested improved production micropropagated valuable anti-diabetic current reveals rate, cost effective conservation technique its use agent at commercial level.