Review of drug analysis with direct serum injection on the HPLC column

摘要: Abstract Contrary to the expectations of past decade, routine therapeutic drug monitoring by HPLC failed expand. Although more extensive perform, immunoassays have dominated market for analysis in last few years. This is attributed lengthy sample preparation needed HPLC. However, remains main method uncommon drugs and their metabolites. The direct serum injection on column simplifies as an analytical technique enables full automation. Advantages disadvantages this are discussed. review tries summarize different approaches column: 1) Guard columns, 2) Micellar chromatography, 3) Internal surface reversed-phase 4) Column switching, 5) Wide-pore (protein) columns. With injection, pH mobile phase very critical separating choice from endogenous substances found s...