Gas and Liquid Chromatography, Column Selection for, in Drug Analysis

摘要: Within the realm of pharmaceutical drug analyses, two techniques currently dominate field. High-performance liquid chromatography (HPLC) is at forefront, followed somewhat distantly by gas (GC). HPLC more prevalent than GC owing mainly to heat-labile nature majority substances. Still continues play a very active role. In comparison and GC, combined, frequency use other quite small. Perhaps major reason for this that not only involves qualitative quantitative detection targeted species, but also provides medium isolating these analytes from potential interfering impurities prior measurement. The proficiency combination unparalleled any blending means clean up sample detect target compound. These prominent are often complementary, although each has its own strengths limitations. General guidelines given assist chromatographer elect and/or particular separation. Considerations choosing hardware type sizes, solid support bonded phase presented. Particular emphasis placed on latest, which primary importance obtaining rugged methods. reader directed towards optimum selection based compound interest's functional group type, or activity classification (e.g. barbiturate, cardiovascular, etc.). Assistance in developing new methods frequently arises when starting with an existing published method similar structure. This article 514 references in-depth separation information. addition, background knowledge bonded-phase chemistry, chromatographic modes separation, approaches chiral discrimination processes contributed.