Retrograde neuronal tracing with a deletion-mutant rabies virus

摘要: We have constructed a deletion-mutant rabies virus encoding EGFP and find it to be an excellent tool for studying detailed morphology physiology of neurons projecting injection sites within the mammalian brain. The cannot spread beyond initially infected cells yet, unlike other viral vectors, replicates its core them. therefore fluoresce intensely, revealing fine dendritic axonal structure with no background from partially or faintly labeled cells.