N‐ and C‐terminal degradomics: new approaches to reveal biological roles for plant proteases from substrate identification

摘要: Proteolysis is an irreversible post-translational modification that regulates many intra- and intercellular processes, including essential go/no-go decisions during cell proliferation, development death. Hundreds of protease-coding genes have been identified in plants, but few linked to specific substrates. Conversely, proteolytic processes are frequently observed plant biology rarely they ascribed proteases. In mammalian systems, unbiased system-wide proteomics analyses protease activities recently tremendously successful the identification substrate repertoires, also known as degradomes. Knowledge degradome key understand role proteases vivo. Quantitative shotgun proteomic studies identifying substrates, while simple perform biased toward abundant proteins do not reveal precise cleavage sites. Current degradomics techniques overcome these limitations by focusing on information-rich amino- carboxy-terminal peptides original mature protease-generated neo-termini. Targeted quantitative analysis protein termini identifies sites substrates with exquisite sensitivity dynamic range vitro vivo systems. This review provides overview state-of-the-art methods for enrichment terminal peptides, their application research. These emerging promise clarify elusive biological roles proteolysis plants.