Phosphatidylcholine Biosynthesis during Neuronal Differentiation and Its Role in Cell Fate Determination
作者： Hebe Marcucci , Luciana Paoletti , Suzanne Jackowski , Claudia Banchio
关键词:
摘要: Neuronal differentiation is characterized by neuritogenesis and neurite outgrowth, processes that are dependent on membrane biosynthesis. Thus, the production of phosphatidylcholine (PtdCho), major phospholipid, should be stimulated during neuronal differentiation. We demonstrate retinoic acid (RA)-induced Neuro-2a cells, PtdCho synthesis was promoted an ordered sequential activation choline kinase α (CKα) cytidylyltransferase (CCTα). Early after RA stimulation, increase in mainly governed biochemical CCTα. Later, transcription CKα- CCTα-encoding genes induced. Both biosynthesis ERK activation. A novel mechanism proposed which coordinated Enforced expression either CKα or CCTα increased rate amount PtdCho, these cells initiated without as evidenced cell morphology associated with neuritogenesis. The resulting from enforced persistent These results indicate elevated could mimic signals thus determine fate. Moreover, they explain key role plays regeneration.
sci-hub.se 参考文章(61)
J P Infante, J E Kinsella, Control of phosphatidylcholine synthesis and the regulatory role of choline kinase in rat liver. Evidence from essential-fatty acid-deficient rats Biochemical Journal. ,vol. 176, pp. 631- 633 ,(1978) , 10.1042/BJ1760631
R. Harel, A.H. Futerman, Inhibition of sphingolipid synthesis affects axonal outgrowth in cultured hippocampal neurons Journal of Biological Chemistry. ,vol. 268, pp. 14476- 14481 ,(1993) , 10.1016/S0021-9258(19)85263-8
E Posse de Chaves, D E Vance, R B Campenot, J E Vance, Axonal synthesis of phosphatidylcholine is required for normal axonal growth in rat sympathetic neurons. Journal of Cell Biology. ,vol. 128, pp. 913- 918 ,(1995) , 10.1083/JCB.128.5.913
Pierre Chambon, A decade of molecular biology of retinoic acid receptors. The FASEB Journal. ,vol. 10, pp. 940- 954 ,(1996) , 10.1096/FASEBJ.10.9.8801176
F. Dehaut, I. Bertrand, T. Miltaud, A. Pouplard-Barthelaix, M. Maingault, N-6 polyunsaturated fatty acids increase the neurite length of PC12 cells and embryonic chick motoneurons Neuroscience Letters. ,vol. 161, pp. 133- 136 ,(1993) , 10.1016/0304-3940(93)90277-R
Dennis E. Vance, Christopher J. Walkey, Roles for the methylation of phosphatidylethanolamine. Current Opinion in Lipidology. ,vol. 9, pp. 125- 130 ,(1998) , 10.1097/00041433-199804000-00008
Joseph R. Marszalek, Claire Kitidis, Ariya Dararutana, Harvey F. Lodish, Acyl-CoA synthetase 2 overexpression enhances fatty acid internalization and neurite outgrowth. Journal of Biological Chemistry. ,vol. 279, pp. 23882- 23891 ,(2004) , 10.1074/JBC.M313460200
W. Araki, R. J. Wurtman, Control of membrane phosphatidylcholine biosynthesis by diacylglycerol levels in neuronal cells undergoing neurite outgrowth Proceedings of the National Academy of Sciences of the United States of America. ,vol. 94, pp. 11946- 11950 ,(1997) , 10.1073/PNAS.94.22.11946
Neil Sidell, Adrienne Altman, Mark R. Haussler, Robert C. Seeger, Effects of retinoic acid (RA) on the growth and phenotypic expression of several human neuroblastoma cell lines Experimental Cell Research. ,vol. 148, pp. 21- 30 ,(1983) , 10.1016/0014-4827(83)90184-2
Marica Bakovic, Kristin Waite, Wei Tang, Ira Tabas, Dennis E. Vance, Transcriptional activation of the murine CTP:phosphocholine cytidylyltransferase gene (Ctpct): combined action of upstream stimulatory and inhibitory cis-acting elements Biochimica et Biophysica Acta. ,vol. 1438, pp. 147- 165 ,(1999) , 10.1016/S1388-1981(99)00042-6