Brain proline-directed protein kinase is a neurofilament kinase which displays high sequence homology to p34cdc2.

摘要: The carboxyl-terminal regions of neurofilament high (NF-H) and middle (NF-M) molecular weight proteins have been suggested to be phosphorylated in vivo by a p34cdc2-like protein kinase, on the basis phosphorylation site motif vitro p34cdc2 kinase (Hisanaga, S.I., Kusubata, M., Okumura, E. Kishimoto, T. (1991) J. Biol. Chem. 266, 21798-21803). A novel proline-directed previously identified purified from bovine brain has found this study phosphorylate NF-H NF-M at sites identical those HeLa cell kinase. is composed 33-kDa 25-kDa subunit. subunit was partially sequenced, degenerate oligonucleotide primers corresponding amino acid sequence information were used clone polymerase chain reaction (PCR). Two overlapping PCR products comprised complete open reading frame 292 acids. contains all features suggesting that peptide represents catalytic shows approximately equal homology human cdk2, with about 58 59% identity, respectively. These results suggest new category cdc2 family, some members family may major functions unrelated cycle control.