Methylation of the p73 gene in gliomas.

摘要: The p73 gene encodes a protein structurally and functionally homologous to TP53, maps chromosomal band 1p36.33, where loss of heterozygosity has been observed in up 90% oligodendrogliomas 10-25% diffuse astrocytomas. We assessed the methylation status CpG islands promoter region by methylation-specific PCR 117 glioma biopsies. Methylation was detected 5 out 28 (18%) glioblastomas 4/26 (15%) anaplastic (WHO grade III) but not II oligodendrogliomas, low-grade astrocytomas (grade II), III). To assess whether leads expression, we carried reverse transcription-PCR 10 glioblastoma cell lines. Two lines (U87MG T98G) showed methylation. U87MG had no unmethylated (complete methylation), expression. T98G methylated (partial retained A third line (LN-308) expression without These results suggest that complete is associated with additional mechanisms may cause Analysis polymorphic site exon 2 further mono-allelically expressed 6 7 primary gliomas heterozygous GC/TA polymorphism.