Design Principles for Riboswitch Function

摘要: Scientific and technological advances that enable the tuning of integrated regulatory components to match network system requirements are critical reliably control function biological systems. RNA provides a promising building block for construction tunable based on its rich capacity our current understanding sequence–function relationship. One prominent example RNA-based is riboswitches, genetic elements mediate ligand gene expression through diverse mechanisms. While characterization natural synthetic riboswitches has revealed riboswitch can be modulated sequence alteration, no quantitative frameworks exist investigate or guide tuning. Here, we combined mathematical modeling experimental approaches relationship between performance. Model results demonstrated competition reversible irreversible rate constants dictates performance different We also found practical restrictions, such as an upper limit concentration, significantly alter performance, necessitating alternative strategies. Previous data well experiments conducted in this work support model predictions. From results, developed set general design principles riboswitches. Our provide foundation from which how tuned meet systems-level demands.