emission tomography imaging of rheumatoid arthritis in an animal model

摘要: Introduction: Rheumatoid arthritis (RA) is a chronic disease, affecting 0.5 to 1% of adults in industrialized countries, which systemic inflammation and synovitis drive joint destruction. [ 18 F]DPA-714 specific tracer the kDa translocator protein (TSPO), overexpressed on activated macrophages, proposed as biomarker neuroinflammation. Today, diagnosis patients with early inflammatory limited by poor sensitivity specificity. The present study aims investigate potential monitor vivo processes at preclinical stage via positron emission tomography (PET). Methods: RA was induced Dark Agouti rats subcutaneous injection inactivated Mycobacterium tuberculosis. Development clinical signs investigated daily severity disease evaluated. Animals were imaged peak using small-animal PET-CT tomograph. Results: first appeared 10 days post-injection, 20 days. At this time, PET-analyses showed clear uptake swollen ankles, mean values 0.52 ± 0.18% injected dose (ID/cc) for treated (n= 11) 0.19 0.09 non-treated 6) rats. A good correlation between F]DPA-714’s swelling also found. Immunohistochemistry an enhanced TSPO expression hind paws, mainly co-localized macrophages antigen CD68 expressing cells. Conclusion: These preliminary results demonstrate that 18kDa radioligand adapted follow-up linked our experimental model, suggesting strong imaging peripheral inflammation.