Probing yeast molecular systems biology by new proteomics strategies

摘要: The characterization of protein architecture in complex cellular model systems such asthe unicellular yeast Saccharomyces cerevisiae has become one the major biological challenges last decades.. development high throughput methodology to identify and quantify proteins from samples, mainly using mass spectrometry, however enabled us stitch concepts together into a more global perspective. Proteins are that sense remarkable molecules since their interactions, modifications catalytic functions give rise sheer endless amount networks. Moreover, we now begin understand often do not just have particular function but participate wide range processes. In order obtain information on characteristics as stability, interactions there is need develop dedicated analytical methods focus each aspect, for instance phosphorylation. integration multiple levels gained this way will lead better understanding functioning.