[12] Mass spectrometry of peptides and proteins by matrix-assisted ultraviolet laser desorption/ionization

摘要: Publisher Summary This chapter discusses mass spectrometry of peptides and proteins by matrix-assisted ultraviolet laser desorption/ionization. Three features make an attractive analytical technique for biorganic molecules: very high sensitivity, potential highly accurate determination, ability to elucidate structural information the molecules under investigation. It is shown in this that goal extremely sensitivity has already been achieved desorption/ionization (LDI-MS). also yields important information—namely, on level quaternary and, possibly, tertiary structures more than primary secondary ones. The attainable accuracy molecular determination still leaves much be desired, but substantial improvements can expected. Most lasers throughout wavelength range from far-ultraviolet far-infrared with pulse durations continuous wave down femtosecond (10 -15 sec) have at some point used irradiate solid or liquid material vacuum ambient pressure intent couple energy beam into sample via linear nonlinear absorption transfer gas phase, a prerequisite spectrometric analysis.