Ultraviolet-laser desorption/ionization mass spectrometry of femtomolar amounts of large proteins
作者： M. Karas , A. Ingendoh , U. Bahr , F. Hillenkamp
关键词: Chemistry 、 Sample preparation in mass spectrometry 、 Quantitative analysis (chemistry) 、 Analytical chemistry 、 Protein mass spectrometry 、 Mass spectrometry 、 Molecular mass 、 Chromatography 、 Ultraviolet 、 Ion 、 Desorption 、 Molecular medicine 、 Biochemistry 、 Pollution
摘要: Proteins with molecular weights between 14 000 and 175 daltons were investigated by matrix-assisted laser desorption/ionization mass spectrometry. High abundance ions obtained absolute sample amounts ranging from 5 pmol down to 50 fmol, allowing weight determination an accuracy 0.1 0.2%. The amount of needed for analysis is determined the preparation rather than step; majority can be regained after analysis.
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