Optimization of sample preparation for plasma desorption mass spectrometry of peptides and proteins using a nitrocellulose matrix
作者： Per F. Nielsen , Klaus Klarskov , Peter Højrup , Peter Roepstorff
关键词:
摘要: Molecular ion yields and spectrum quality in plasma desorption mass spectrometry of peptides proteins have been studied as a function the method sample preparation. Parameters like matrix preparation, pH solution, applying on quantity studied. The best was found to be nitrocellulose prepared by electrospraying. Various techniques for result different sensitivity. highest sensitivity is obtained using spin-drying technique, which results high-quality spectra with 1–2 pmol trypsin (mol. wt 23,463). preparation are discussed directions an optimal procedure given.
sci-hub.se 参考文章(12)
J Mikkelsen, P Højrup, P F Nielsen, P Roepstorff, J Knudsen, Amino acid sequence of acyl-CoA-binding protein from cow liver. Biochemical Journal. ,vol. 245, pp. 857- 861 ,(1987) , 10.1042/BJ2450857
P. Roepstorff, P.F. Nielsen, B.U.R. Sundqvist, P. Håkansson, G. Jonsson, The influence of sample preparation on molecular ion formation in plasma desorption mass spectrometry of peptides and proteins International Journal of Mass Spectrometry and Ion Processes. ,vol. 78, pp. 229- 236 ,(1987) , 10.1016/0168-1176(87)87051-9
P. Roepstorff, P. Højrup, B. U. R. Sundqvist, G. Jonsson, P. Håkansson, S. O. Andersen, K. E. Johansson, Application of plama desorption mass spectrometry to molecular weight determination of structural protein from insect cuticle Journal of Mass Spectrometry. ,vol. 13, pp. 689- 691 ,(1986) , 10.1002/BMS.1200131209
A. Hedin, P. Håkansson, B.U.R. Sundqvist, A comparison of fast heavy ion induced desorption of biomolecules from multilayer films and monolayers adsorbed to nitrocellulose backings Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms. ,vol. 22, pp. 491- 496 ,(1987) , 10.1016/0168-583X(87)90147-9
Gunnar P. Jonsson, Allan B. Hedin, Per L. Hakansson, Bo U. R. Sundqvist, B. Goeran S. Saeve, Per F. Nielsen, Peter. Roepstorff, Karl Erik. Johansson, Ivan. Kamensky, Maria S. L. Lindberg, Plasma desorption mass spectrometry of peptides and proteins adsorbed on nitrocellulose Analytical Chemistry. ,vol. 58, pp. 1084- 1087 ,(1986) , 10.1021/AC00297A023
E.A. Jordan, R.D. Macfarlane, C.R. Martin, C.J. McNeal, Ion-containing polymers: A new matrix for 252Cf-plasma desorption mass spectrometry International Journal of Mass Spectrometry and Ion Physics. ,vol. 53, pp. 345- 348 ,(1983) , 10.1016/0020-7381(83)85128-6
C.J. Van Oss, R.J. Good, M.K. Chaudhury, Mechanism of DNA (Southern) and protein (Western) blotting on cellulose nitrate and other membranes. Journal of Chromatography A. ,vol. 391, pp. 53- 65 ,(1987) , 10.1016/S0021-9673(01)94304-3
B. Sundqvist, I. Kamensky, P. Håkansson, J. Kjellberg, M. Salehpour, S. Widdiyasekera, J. Fohlman, P. A. Peterson, P. Roepstorff, Californium-252 plasma desorption time of flight mass spectroscopy of proteins Biological Mass Spectrometry. ,vol. 11, pp. 242- 257 ,(1984) , 10.1002/BMS.1200110509
Ronald D. Macfarlane, Catherine J. McNeal, Charles R. Martin, Mass spectrometric study of ion adsorption on poly(ethylene terephthalate) and polypropylene surfaces Analytical Chemistry. ,vol. 58, pp. 1091- 1097 ,(1986) , 10.1021/AC00297A025
C. J. NcNeal, R. D. Macfarlane, E. L. Thurston, Thin film deposition by the electrospray method for californium-252 plasma desorption studies of involatile molecules Analytical Chemistry. ,vol. 51, pp. 2036- 2039 ,(1979) , 10.1021/AC50048A032