Direct cytotoxicity of ethylcholine mustard aziridinium in cerebral microvascular endothelial cells.
作者： Carmen Gómez , Carmen Martín , Elena Galea , Carmen Estrada
DOI: 10.1111/J.1471-4159.1993.TB03318.X
关键词:
摘要: : The choline analogue ethylcholine mustard aziridinium (AF64A) is a potent and irreversible inhibitor of uptake in brain synaptosomes used as neurotoxin to produce animal models cholinergic hypofunction. However, previous studies have shown that intraocular administration AF64A rats not only reduced the number neurons retina, but also induced ultrastructural alterations microvasculature. purpose this study was investigate whether has direct cytotoxic effect on endothelial cells. As revealed by measurement lactate dehydrogenase activity culture medium, produced similar concentration-dependent cellular damage cultures bovine cerebral cells human neuroblastoma cell line SK-N-MC, smooth muscle toxic correlated well with affinity transport system detected each type. toxin mediated its interaction carrier, demonstrated following observations: (a) inhibited [3H]choline manner both cultured freshly isolated cells, (b) addition or hemicholinium-3 medium prevented AF64A-induced toxicity cultures.
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