Nuclear translocation of Nrf2 and expression of antioxidant defence genes in THP-1 cells exposed to carbon nanotubes.
作者： David M. Brown , Kenneth Donaldson , Vicki Stone
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摘要: Carbon nanotubes have a wide range of applications in various industries and their use is likely to rise the future. Currently, major concern that with increasing production these materials, there may be increased health risks exposed workers. Long (> 15 microm) straight undergo frustrated phagocytosis which result reduced clearance. We examine here effects multiwalled carbon different sizes on monocytic THP-1 cells, regard ability stimulate expression HO-1 GST genes produce nuclear translocation transcription factor, Nrf2, as well release several pro-inflammatory cytokines mediators inflammation. Our results suggest long (50 (62.5 microg/ml for 4 hours) Nrf2 gene compared shorter entangled nanotubes. There was no GST. The (NT1) caused proinflammatory cytokine IL-1beta, an effect diminished by antioxidant trolox, suggesting role oxidative stress upregulation this cytokine. Tentatively, our study suggests exert cells part via mechanism.
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researchgate.net 参考文章(10)
V. Stone, J. Shaw, D.M. Brown, W. MacNee, S.P. Faux, K. Donaldson, The role of oxidative stress in the prolonged inhibitory effect of ultrafine carbon black on epithelial cell function. Toxicology in Vitro. ,vol. 12, pp. 649- 659 ,(1998) , 10.1016/S0887-2333(98)00050-2
D.M. Brown, I.A. Kinloch, U. Bangert, A.H. Windle, D.M. Walter, G.S. Walker, C.A. Scotchford, K. Donaldson, V. Stone, An in vitro study of the potential of carbon nanotubes and nanofibres to induce inflammatory mediators and frustrated phagocytosis Carbon. ,vol. 45, pp. 1743- 1756 ,(2007) , 10.1016/J.CARBON.2007.05.011
Sivaram Arepalli, Pavel Nikolaev, William Holmes, Bradley S. Files, Production and measurements of individual single-wall nanotubes and small ropes of carbon Applied Physics Letters. ,vol. 78, pp. 1610- 1612 ,(2001) , 10.1063/1.1352659
Martin J.D. Clift, Barbara Rothen-Rutishauser, David M. Brown, Rodger Duffin, Ken Donaldson, Lorna Proudfoot, Keith Guy, Vicki Stone, The impact of different nanoparticle surface chemistry and size on uptake and toxicity in a murine macrophage cell line. Toxicology and Applied Pharmacology. ,vol. 232, pp. 418- 427 ,(2008) , 10.1016/J.TAAP.2008.06.009
M. W. Pfaffl, A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Research. ,vol. 29, pp. 0- 0 ,(2001) , 10.1093/NAR/29.9.E45
Craig A. Poland, Rodger Duffin, Ian Kinloch, Andrew Maynard, William A. H. Wallace, Anthony Seaton, Vicki Stone, Simon Brown, William MacNee, Ken Donaldson, Carbon nanotubes introduced into the abdominal cavity of mice show asbestos-like pathogenicity in a pilot study. Nature Nanotechnology. ,vol. 3, pp. 423- 428 ,(2008) , 10.1038/NNANO.2008.111
Truyen Nguyen, Philip J. Sherratt, Cecil B. Pickett, Regulatory Mechanisms Controlling Gene Expression Mediated by the Antioxidant Response Element Annual Review of Pharmacology and Toxicology. ,vol. 43, pp. 233- 260 ,(2003) , 10.1146/ANNUREV.PHARMTOX.43.100901.140229
Sharmistha Pal, Romy Yun, Antara Datta, Lynne Lacomis, Hediye Erdjument-Bromage, Jitendra Kumar, Paul Tempst, Saïd Sif, None, mSin3A/histone deacetylase 2- and PRMT5-containing Brg1 complex is involved in transcriptional repression of the Myc target gene cad. Molecular and Cellular Biology. ,vol. 23, pp. 7475- 7487 ,(2003) , 10.1128/MCB.23.21.7475-7487.2003
I. Rahman, W. MacNee, Role of transcription factors in inflammatory lung diseases Thorax. ,vol. 53, pp. 601- 612 ,(1998) , 10.1136/THX.53.7.601
Ralf Schreck, Kaj Albermann, Patrick A. Baeuerle, Nuclear Factor Kb: An Oxidative Stress-Responsive Transcription Factor of Eukaryotic Cells (A Review) Free Radical Research Communications. ,vol. 17, pp. 221- 237 ,(1992) , 10.3109/10715769209079515