The effect of ascorbic acid on the synthesis of collagen precursor secreted by 3T6 mouse fibroblasts in culture

摘要: 1. 1.|Although the soluble collagen secreted into growth medium by 3T6 mouse fibroblasts was substantially underhydroxylated in absence of ascorbic acid, there no significant reduction amount secreted, during 24 h, nor an increase degradation to small fragments, comparison with fully hydroxylated material from ascorbate-supplemented cultures. 2. 2.|Studies based on agarose gel filtration a variety denaturing solvents indicate difference stability between large complex obtained cultures, and that deficient cultures. In addition, cultures yielded relatively more Pro-α chains, while supplemented α chains. 3. 3.|Studies disc electrophoresis carboxymethyl cellulose chromatography also indicated ascorbate-deficient yield when compared cultures. 4. 4.|When subjected pepsin digestion followed thermal denaturation, aggregates chains disappeared, leaving only. More could be recovered than -deficient cultures. 5. 5.|Trichloroacetic acid-ethanol fractionation studies confirmed abnormal solubility properties medium, better both before after treatment. 6. 6.|It is proposed precursor produced may not converted as readily insoluble