Intracellular-diced dsRNA has enhanced efficacy for silencing HCV RNA and overcomes variation in the viral genotype.

摘要: RNA interference (RNAi) can be used to inhibit viral replication in mammalian cells and therefore could a powerful new antiviral therapy. Small interfering (siRNA) may effective for RNAi, but there are some technical problems that must solved each case, example, predicting the siRNA target site targeting heterogeneous sequences virus population. We show here diced generated from long double-stranded (dsRNA) is highly inducing RNAi HuH-7 harboring hepatitis C (HCV) replicons overcome variations HCV genotype. However, cells, dsRNA induced an interferon response caused cell death. Here we describe improvement of this method, U6 promoter-driven expression hairpin-RNA with multiple point mutations sense strand. This efficiently silence protein without triggering or death normally by dsRNA. In conclusion, intracellular-diced induces and, despite high rate mutation HCV, it should feasible therapeutic strategy silencing RNA.