Specific strains of Escherichia coli are pathogenic for the endometrium of cattle and cause pelvic inflammatory disease in cattle and mice.

摘要: Background Escherichia coli are widespread in the environment and pathogenic strains cause diseases of mucosal surfaces including female genital tract. Pelvic inflammatory disease (PID; metritis) or endometritis affects ∼40% cattle after parturition. We tested expectation that multiple genetically diverse E. from opportunistically contaminate uterine lumen parturition to establish PID. Methodology/Principal Findings Distinct clonal groups were identified by Random Amplification Polymorphic DNA (RAPD) Multilocus sequence typing (MLST) animals with these differed known diarrhoeic extra-intestinal coli. The endometrial (EnPEC) more adherent invasive for epithelial stromal cells, compared isolated uterus clinically unaffected animals. cells produced prostaglandin E2 interleukin-8 response lipopolysaccharide (LPS) purified EnPEC non-pathogenic their LPS also caused PID when infused into mice accumulation neutrophils macrophages endometrium. Infusion was only associated bacterial invasion endometrium myometrium. Despite ability invade cultured elicit host cell responses PID, lacked sixteen genes commonly adhesion enteric extraintestinal coli, though ferric yersiniabactin uptake gene (fyuA) present PID-associated EnPEC. Endometrial wild type but not Toll-like receptor 4 (TLR4) null secreted chemokine (C-X-C motif) ligand 1 (CXCL1) EnPEC, highlighting key role PID. Conclusions/Significance The implication arising discovery is development treatments vaccines should focus specifically on other