Minimum inhibitory concentrations of selected antimicrobials against Escherichia coli and Trueperella pyogenes of bovine uterine origin.

摘要: Minimum inhibitory concentrations (MIC) of 9 antimicrobials for isolates 2 common bovine intrauterine bacterial pathogens, Escherichia coli (n=209) and Trueperella pyogenes (n=35), were determined using broth microdilution methodology. The recovered from dairy cows 7 herds postpartum the cytobrush technique. pathogens initially identified phenotypic techniques. Additionally, PCR was used to confirm identity T. categorize E. into phylogenetic groups A, B1, B2, D. in excess published cut-points or bimodal distributions MIC indicated potential antimicrobial resistance ampicillin, cefuroxime, cephapirin, oxytetracycline coli, pyogenes. Of tested, ticarcillin/clavulanic acid, ceftiofur, enrofloxacin had lowest these pathogens. Differences some found between herds, age, breeds, groups. Isolation with an ≥8μg/mL at 23d associated a lower probability pregnancy within 6wk commencement breeding compared those <8μg/mL (relative risk=0.66). uterine New Zealand cows. However, absence either epidemiological clinical interpretive criteria, interpretation remains unclear. Further studies are required define interpretative including determination pharmacokinetic pharmacodynamic profiles antimicrobials.