Cell-free synthesis of mouse corticotropin. Evidence for two high molecular weight gene products.
作者： R W Harrison , D N Orth , M A Haralson , W E Nicholson , S J Fairfield
DOI: 10.1016/S0021-9258(17)30199-0
关键词:
摘要: Abstract Polysomes or mRNA prepared from cultured AtT-20/D16v mouse pituitary adenocarcinoma cells direct the efficient incorporation of amino acid into newly synthesized material in presence wheat germ translational factors. A significant franction total cell-free product is specifically immunoprecipitable with corticotropin antibody purified by immune affinity chromatography. Analysis immunoreactive products sodium dodecyl sulfate-polyacrylamide gel electrophoresis reveals that two high molecular weight species (Mr congruent to 32,500 and 28,000) are an approximate 2:1 ratio. Neither contains carbohydrate based upon concanavalin chromatography exposure polysaccharidases. The smaller does not appear arise proteolytic processing since both approximately same ratio reaction mixtures directed either polysomes mRNA. These results suggest contain distinct poluations specifying synthesis different forms corticotropin.
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