Identification and inactivation of pleiotropic regulator CcpA to eliminate glucose repression of xylose utilization in Clostridium acetobutylicum.
作者： Cong Ren , Yang Gu , Shiyuan Hu , Yan Wu , Pan Wang
DOI: 10.1016/J.YMBEN.2010.05.002
关键词:
摘要: Abstract d -xylose utilization is a key issue for lignocellulosic biomass fermentation, and major problem in this process carbon catabolite repression (CCR). In investigation, solvent-producing bacterium Clostridium acetobutylicum ATCC 824 was metabolically engineered to eliminate -glucose of utilization. The ccpA gene, encoding the pleiotropic regulator CcpA, experimentally characterized then disrupted . Under pH-controlled conditions, -disrupted mutant (824ccpA) can use mixture simultaneously without CCR. Moreover, strain produced acetone, butanol ethanol (ABE) at maximal titer 4.94, 12.05 1.04 g/L, respectively, which close solvent level maize- or molasses-based fermentation by wild type C. Molar balance analysis improved mixed sugars also revealed less acid accumulation more yield as compared type. This study offers genetic modification strategy improving simultaneous , essential commercial exploitation lignocellulose production solvents biofuels.
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