Preparative Scale Chromatography with Analytical Columns

摘要: Two major problems are encountered when using large samples in gas chromatographic separations. First, the charge has to be placed on column such a manner that serious band-broadening does not occur due slow volatilization of sample. Secondly, should operated so peak asymmetry, resulting from non-linear nature adsorption isotherm at high levels concentration, is eliminated or counteracted.