Influence of fatty acids on the binding of calcium to human albumin. Correlation of binding and conformation studies and evidence for distinct differences between unsaturated fatty acids and saturated fatty acids.

摘要: The influence of various fatty acids 14-18 carbon chain lengths on the binding calcium to human serum albumin was studied. cis-unsaturated (myristoleic, palmitoleic, oleic, and linoleic) caused a much larger increase in affinity maximum for than did saturated (myristic, palmitic, stearic). For unsaturated acids, calcium-albumin increased as degree unsaturation or length decreased. With oleic linoleic acid, increases were observed at approximately 2-3 mol acid/mol albumin, while myristic, elaidic concentrations 5-7 acids/mol required. fluorescence emission found change parallel with acids. We conclude that 1) trans-unsaturated have very different effects 2) alteration is likely due conformational molecule, 3) changes are levels which obtainable vivo.