Free Fatty Acid Effects on Mitochondrial Permeability: An Overview

摘要: Abstract A variety of experimental conditions elicit increases in mitochondrial permeability that can be differentiated from the classic cyclosporin (CsA)-sensitive transition (MPT). For example, butylated hydroxytoluene, signal peptides, and hormone thyroxine have been shown to promote are CsA-insensitive. Our laboratory has recently demonstrated palmitic acid, a saturated 16-carbon free fatty acid (FFA), also open CsA-insensitive pore. This nonclassic (NCPT) is further distinguished by nonselective dependence on divalent cations spontaneous closure. To determine if induction NCPT specific resolve conflicting reports as mechanisms which FFAs alter permeability, we examined detail swelling induced differ chain length degree saturation. The following results were obtained: (1) In presence modest Ca2+ concentrations (75 nmol/mg protein), medium-chain (C12–C18) more effective eliciting than shorter or longer FFAs; alkanols amines had no effect. (2) Under these conditions, with all characteristics NCPT, while unsaturated triggered MPT. (3) When matrix concentration was elevated, NCPT. (4) Mitochondrial inhibited but not other alkanols. These suggest ambient greatly influence nature increase FFAs. They consistent our earlier proposal (or Sr2+) binding inner leaflet membrane underlies