Characterization of embryonic cortical neuron death in prolonged cell suspension.

摘要: Cell transplantation may be an effective therapeutic strategy for many neurodegenerative diseases. However, difficulty in obtaining a sufficient amount of donor cells and low graft survival are two major limiting factors. Dissociation from tissues or culture is inevitable step cell transplantation, viability suspension influence the outcome therapy. To this end, we asked whether time freshly dissociated neurons vitro affects their viability. Following 4-24h suspension, primary cortical underwent death. Interestingly, exhibited only marginal caspase-3 immunoreactivity with very few sub-G1 apoptotic proportions flow cytometry. In addition, suppression Bax action failed to prevent death neurons, indicating minimal On other hand, there was marked increase TdT-mediated dUTP nick end labeling-positive propidium iodide-labeled necrotic (∼50%) enhanced poly [ADP-ribose] polymerase-1 activity. Therefore, prevention against necrosis rather than apoptosis required optimal benefits transplantation.