Evaluation and routine application of the novel restricted-access precolumn packing material Alkyl-Diol Silica: coupled-column high-performance liquid chromatographic analysis of the photoreactive drug 8-methoxypsoralen in plasma.

摘要: A fully automated coupled-column HPLC method for on-line sample processing and determination of the photoreactive drug 8-methoxypsoralen (8-MOP) in plasma has been developed. The is based on novel internal-surface reversed-phase precolumn packing materials Alkyl-Diol Silica (ADS). This new family restricted-access a hydrophilic, electroneutral outer particle surface hydrophobic internal pore surface. supports tolerate direct repetitive injection proteinaceous fluids such as allow classical C18-, C8- or C4-reversed-phase partitioning at (pore) total protein load, i.e. lifetime used this study (C8-Alkyl-Diol Silica, 25 μm, × 4 mm I.D.), exceeds more than 100 ml plasma. 8-MOP was detected by its native fluorescence (excitation 312 nm, emission 540 nm). Validation revealed quantitative matrix-independent recovery (99.5–101.3% measured five concentrations between 21.3 625.2 ng per milliliter plasma), linearity over wide range (1.2–3070 8-MOP/ml, r = 0.999), low limits detection (0.39 8-MOP/ml) quantitation (0.79 high between-run (C.V. 1.47%, n 10) within-run 1.33%, reproductivity. paper introduces suitable on-site analysis profiles (bedside-monitoring).