Long noncoding RNA PCAT1 regulates extrahepatic cholangiocarcinoma progression via the Wnt/β-catenin-signaling pathway

摘要: Extrahepatic cholangiocarcinoma (ECC) is a deadly disease that often responds poorly to conventional chemotherapy or radiotherapy. Long noncoding RNAs (lncRNAs) play important roles in human cancers, including ECC, and recent studies indicated the lncRNA prostate cancer-associated transcript 1 (non-protein coding) (PCAT1) involved multiple cancers. However, role of PCAT1 ECC unclear. Previously, we showed up-regulated both tissue samples cell lines. Here, downregulation following transfection with silencing RNA reduced growth increased apoptosis. Additionally, suppression inhibited migration invasion as determined by transwell assay. Furthermore, competing endogenous for microRNA (miR)-122, bioinformatics analysis luciferase-reporter assay results demonstrating regulated WNT1 expression via miR-122. Moreover, levels glycogen synthase kinase 3β significantly decreased β-catenin whole lysates nuclear fractions, indicating Wnt/β-catenin-signaling pathway. We also observed exogenous reversed PCAT1-silencing-induced inhibition inhibition. These progression reducing Wnt/β-catenin signaling through miR-122 repression expression. Our findings revealed an suggested might be potential ECC-related therapeutic target.