Sodium nitroprusside enhances the elicitation power of methyl jasmonate for ginsenoside production in Panax ginseng roots

摘要: The effects of methyl jasmonate (MJ) and sodium nitroprusside (SNP) on ginsenoside production were investigated in ginseng adventitious roots. role nitric oxide (NO) reactive oxygen species (ROS) was also elucidated. Different concentrations SNP applied to highest accumulation recorded for 200 μM SNP. Ginsenoside biosynthesis-related genes highly induced by 100 μM MJ combination with compared MJ alone , which subsequently led higher after 72 h. For the first time, a full length NO-associated (NOA) cDNA clone isolated characterized from embryogenic callus predicted localization mitochondria. open reading frame 1626 bp deduced amino acid sequence 541 residues, shared high degree homology NO associated protein Solanum tuberosum. PgNOA showed transcript levels treated roots combined alone. Increased observed addition MJ-treated samples. There no difference lipoxygenase, key enzyme jasmonic pathway MJ- SNP-treated superoxide dismutase 1 (SOD1) gene responsive treatment SOD1 catalyzes dismutation toxic radicals into either molecular or hydrogen peroxide (H2O2) resulting these products. H2O2 can be converted water action ascorbate peroxidase (APX), expressed results obtained here suggest that enhanced elicitation power increased accumulation. Moreover, this process mediated complex signal transduction network including biosynthesis ROS generation.