Yeast protein expression profile during acetic acid-induced apoptosis indicates causal involvement of the TOR pathway.

摘要: Although acetic acid has been shown to induce apoptosis in yeast, the exact apoptotic mechanisms remain unknown. Here, we studied effects of treatment on yeast cells by 2-DE, revealing alterations levels proteins directly or indirectly linked with target rapamycin (TOR) pathway: amino-acid biosynthesis, transcription/translation machinery, carbohydrate metabolism, nucleotide stress response, protein turnover and cell cycle. The increased involved biosynthesis presented a counteracting response severe intracellular starvation induced acid. Deletion GCN4 GCN2 encoding key players general control (GAAC) system caused higher resistance indicating an involvement Gcn4p/Gcn2p signaling. Involvement TOR pathway acid-induced was also reflected survival rates associated terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)-negative phenotype lower reactive oxygen species Deltator1 cells. In addition, deletion mutants for several downstream mediators revealed that signaling involves phosphatases Pph21p Pph22p but not Sit4p. Altogether, our results indicate GAAC pathways (Tor1p) are apoptosis.