Genome-Based Comparative Analyses of Antarctic and Temperate Species of Paenibacillus

摘要: Antarctic soils represent a unique environment characterised by extremes of temperature, salinity, elevated UV radiation, low nutrient and water content. Despite the harshness this environment, members 15 bacterial phyla have been identified in Ross Sea Region (RSR). However, survival mechanisms ecological roles these are largely unknown. The aim study was to investigate whether strains Paenibacillus darwinianus owe their resilience substantial genomic changes. For this, genome-based comparative analyses were performed on three P. strains, isolated from gamma-irradiated RSR soils, together with nine temperate, soil-dwelling spp. genome each strain sequenced over 1,000-fold coverage, then assembled into contigs totalling approximately 3 Mbp per genome. Based occurrence essential, single-copy genes, completeness estimated at 88%. Genome analysis revealed between 3,043–3,091 protein-coding sequences (CDSs), primarily associated two-component systems, sigma factors, transporters, sporulation genes induced cold-shock, oxidative osmotic stresses. These provide an insight metabolic potential darwinianus, revealing adaptive for soils. large proportion also temperate spp., suggesting that beneficial growth range soil environments. genomes contain significantly fewer CDSs lower paralogous Notwithstanding incompleteness assemblies, differences sizes, determined number clusters CDS content, indicative content scaling. Finally, resource further investigations expression physiological attributes enable under extreme conditions selection processes affect prokaryotic evolution.