Effects of rabbit pinna-derived blastema cells on tendon healing

摘要: Objectives Tendon healing is substantially slow and often associated with suboptimal repair. Cell therapy one of the promising methods to improve tendon Blastema, a population undifferentiated cells, represents characteristics pluripotent mesenchymal stem cells has potentials be used in regenerative medicine. The aim this study was investigate use blastema allotransplantation rabbit healing. Materials In study, as donor, twenty-four rabbits were divided into control treatment groups. Blastema obtained from ear pinna upon punch hole injury donor rabbit. Under general anesthesia, complete transverse tenotomy performed on midsubstance deep digital flexor followed by suture-repair. group, 1 × 106 suspended buffer saline injected intratendinously at repair site, while group received only saline. Cast coaptation maintained for two weeks. Eight weeks after operation, tendons harvested, histopathological, biomechanical, biochemical assays samples. Results Mechanical testing showed significant increase ultimate load, energy absorption, stiffness, yield stress, strain blastema-treated compared controls. Also, higher hydroxyproline content improved collagen alignment along lower inflammatory cell infiltration decreased angiogenesis observed tendons. Conclusion Increased levels histopathological biomechanical parameters suggest that could considered an adjunct rabbits.