Determination of intracellular adenosine triphosphate for detecting anti-HLA antibody-mediated cytolysis. Introduction to a new method for HLA typing.

摘要: Intracellular adenosine triphosphate (ATP) determination appears to be a rapid, and reliable technique for the detection of complement-dependent cytolysis mediated by cytotoxic anti-HLA sera. Only few minutes after addition complement anti-HLA-coated target lymphocytes, striking loss intracellular ATP is observed. The can easily performed with minute amounts cells, serum, complement, exactly following technical conditions standard HLA typing microcytotoxicity test (MCT). One its main advantages over MCT that reading results automatically. Since in most tissue laboratories all steps preceding have already been automated, this method could great interest, view permitting complete automation typing.