Process for determining hl antigens

作者: Christine Treffert , Reiner Dr. Scherer , Karl Dr. Wulff

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摘要: To determine HLA antigens on lymphocytes by incubating the with antiserum and complement liberation of ATP from cells lysed complement, released is reacted an ATPase, then not are quickly determined it. A reagent suitable for this contains a nonionic detergent, luciferin, luciferase, magnesium, complexing agent, apyrase, serum albumin buffer.